Stan Lee
Mayor (voice) (52 eps)
Michelle Trachtenberg
Valkyrie (52 eps)
Shawn Ashmore
Iceman (52 eps)
Tricia Helfer
Sif (52 eps)
Tom Kenny
Iron Man / Captain America / MODOK (voice) (52 eps)
Charlie Adler
Dr. Doom (voice) (52 eps)
Travis Willingham
Hulk (voice) (52 eps)
David Boat
Thor (voice) (52 eps)
Mark Hamill
Chthon (52 eps)
James Marsters
Mister Fantastic (52 eps)
Mikey Kelley
Silver Surfer (voice) (52 eps)
Cree Summer
Storm (voice) (52 eps)
Jennifer Morrison
Wasp (voice) (3 eps)
Nina Dobrev
Ellen (voice) (1 eps)
John Barrowman
Stranger (voice) (1 eps)
Jonathan Frakes
High Evolutionary (voice) (1 eps)