Josh Keaton
Peter Parker / Spider Man (voice) (13 eps)
Joshua LeBar
Flash Thompson / Car Thief #1 (voice) (12 eps)
Lacey Chabert
Gwen Stacy (voice) (12 eps)
Grey DeLisle
Sally Avril / Computer Voice (voice) (10 eps)
Deborah Strang
May Parker (voice) (10 eps)
Phil LaMarr
Rand Robertson / Robbie Robertson (voice) (9 eps)
Ben Diskin
Eddie Brock (voice) (9 eps)
James Arnold Taylor
Harry Osborn / Frederick Foswell (voice) (9 eps)
Daran Norris
J. Jonah Jameson / John Jameson (voice) (8 eps)
Kevin Michael Richardson
Coach Smith (voice) (7 eps)
John DiMaggio
Hammerhead / Car Thief #2 (voice) (7 eps)
Clancy Brown
Rhino / George Stacy (voice) (7 eps)
Vanessa Marshall
Mary Jane Watson (voice) (7 eps)
Alan Rachins
Norman Osborn (voice) (7 eps)
Peter MacNicol
Otto Octavius (voice) (6 eps)
Alanna Ubach
Liz Allan (voice) (6 eps)
Dee Bradley Baker
Curt Connors (voice) (4 eps)
Crispin Freeman
Thug #1 (voice) (3 eps)
Cree Summer
Glory Grant (voice) (2 eps)
Robert Englund
Adrian Toomes / Vulture (voice) (2 eps)
Ed Asner
Ben Parker (voice) (1 eps)
Tricia Helfer
Black Cat (voice) (1 eps)