Benjamin Valic
Peter Parker / Spider-Man (Spidey) (voice) (130 eps)
Lily Sanfelippo
Gwen Stacy / Ghost-Spider (voice) (130 eps)
Jakari Fraser
Miles Morales / Spider-Man (Spin) (voice) (130 eps)
JP Karliak
Norman Osborn / Green Goblin (voice) (36 eps)
Kelly Ohanian
Carolyn Trainer / Doctor Octopus (voice) (32 eps)
Melanie Minichino
Aunt May (voice) (23 eps)
Justin Shenkarow
Aleksei Sytsevich / Rhino (voice) (20 eps)
Stephanie Lemelin
Francine Frye / Electro (voice) (12 eps)
Armen Taylor
Bruce Banner / Hulk (voice) (12 eps)
Dee Bradley Baker
Trace-E (voice) (8 eps)
Tru Valentino
T'Challa / Black Panther (voice) (8 eps)
Sandra Saad
Kamala Khan / Ms. Marvel (voice) (8 eps)
Jaiden Klein
Felicia Hardy / Black Cat (voice) (7 eps)
John Eric Bentley
Mr. Von Carnegie (voice) (7 eps)
Gabrielle Ruiz
Rio Morales (voice) (6 eps)
Kari Wahlgren
Helen Stacy (voice) (6 eps)
John Stamos
Tony Stark / Iron Man (voice) (6 eps)
Eugene Byrd
Jefferson Morales (voice) (6 eps)
Thomas F. Wilson
William Baker / Sandman (voice) (5 eps)
Scott Porter
George Stacy (voice) (4 eps)
Sean Giambrone
Hank Pym / Ant-Man (voice) (3 eps)
Maya Tuttle
Janet Van Dyne / Wasp (voice) (3 eps)
Hoku Ramirez
Humberto Lopez / Reptil (voice) (3 eps)
Bindi Irwin
Isla Coralton (voice) (1 eps)
Sophia Ramos
Gloria Morales (voice) (1 eps)
Melique Berger
Mayor (voice) (1 eps)
Patrick Stump
Concert Announcer (voice) (1 eps)
Hayden Bishop
Jerry (voice) (1 eps)