Christopher Daniel Barnes
Peter Parker / Spider-Man (voice) (11 eps)
Sara Ballantine
Mary Jane Watson (voice) (11 eps)
Jennifer Hale
Felicia Hardy (voice) (11 eps)
Linda Gary
Aunt May Parker (voice) (11 eps)
Gary Imhoff
Harry Osborn (voice) (11 eps)
Ed Asner
J. Jonah Jameson (voice) (11 eps)
Rodney Saulsberry
Joe "Robbie" Robertson (voice) (11 eps)
Roscoe Lee Browne
Kingpin (voice) (11 eps)
Joseph Campanella
Dr. Curt Connors (voice) (4 eps)
Nick Jameson
Michael Morbius (voice) (4 eps)
David Warner
Landon (voice) (4 eps)
Liz Georges
Debra Whitman (voice) (4 eps)
Nita Talbot
Anastasia Hardy (voice) (3 eps)
Bob Bergen
Ned Leeds (voice) (3 eps)
Patrick Labyorteaux
Flash Thompson (voice) (3 eps)
Majel Barrett
Anna Watson (voice) (3 eps)
Oliver Muirhead
Whistler (voice) (2 eps)
Neil Ross
Norman Osborn / Green Goblin (voice) (2 eps)
John Phillip Law
John Hardesky / The Cat (voice) (2 eps)
Gregg Berger
Quentin Beck / Mysterio (voice) (2 eps)
Don Reed
Clay Marks (voice) (2 eps)
Jack Angel
Nick Fury (voice) (2 eps)
Efrem Zimbalist Jr.
Doctor Octopus (voice) (2 eps)
Iona Morris
Martha Robertson (voice) (1 eps)
Nichelle Nichols
Miriam (voice) (1 eps)
Tim Russ
Hobie Brown / Prowler (voice) (1 eps)
John Beck
Frank Castle / Punisher (voice) (1 eps)
Alan Johnson
Arthur Avis / Young Vulture (voice) (1 eps)
Roger Kern
Gecko (voice) (1 eps)
Robert Axelrod
Microchip (voice) (1 eps)
Alfonso Ribeiro
Randy Robertson (voice) (1 eps)
Lawrence Mandley
Iceberg (voice) (1 eps)
Kathy Garver
Gila (voice) (1 eps)
Sandra Bernhard
Sarah Baker (voice) (1 eps)
Nell Carter
Glory Grant (voice) (1 eps)
Stanley Ralph Ross
Joey Nails (voice) (1 eps)
Townsend Coleman
Middle-Aged Silvermane (voice) (1 eps)
Beverly Garland
Miranda Wilson (voice) (1 eps)
Lois Nettleton
Nora (voice) (1 eps)
Frankie Crocker
Jimmy Dugan (voice) (1 eps)
David Hayter
Brace Huntington (voice) (1 eps)
Susan Beaubian
Dr. Mariah Crawford / Calypso (voice) (1 eps)
Giselle Loren
Margaret Connors (voice) (1 eps)
Dorian Harewood
Tombstone (voice) (1 eps)
Cannon Young
Child Silvermane (voice) (1 eps)
Anne-Marie Johnson
Angela (voice) (1 eps)
Eddie Albert
Adrian Toomes / Old Vulture (voice) (1 eps)
Jeff Corey
Silvermane (voice) (1 eps)
Marla Rubinoff
Liz Allen (voice) (1 eps)
Leigh-Allyn Baker
Alisa Silvermane (voice) (1 eps)
John Beard
News Reporter (voice) (1 eps)
Ed Gilbert
Mary Jane's Father (voice) (1 eps)
Jim Cummings
Shocker (voice) (1 eps)
Dawnn Lewis
Detective Terri Lee (voice) (1 eps)
Richard Moll
Scorpion (voice) (1 eps)
Rachel Davies
Agent X (voice) (1 eps)
J.D. Hall
Blade (voice) (1 eps)
Maxwell Caulfield
Alistair Smythe (voice) (1 eps)