Paul Reubens
Pee-wee Herman (45 eps)
Lynne Marie Stewart
Miss Yvonne (45 eps)
Laurence Fishburne
Cowboy Curtis (45 eps)
S. Epatha Merkerson
Reba (45 eps)
John Paragon
Jambi (45 eps)
Alison Mork
Chairry (voice) / Magic Screen (voice) (45 eps)
Suzanne Kent
Mrs. Renee (32 eps)
William Marshall
The King of Cartoons (32 eps)
Phil Hartman
Captain Carl (13 eps)
Gilbert Lewis
The King of Cartoons (13 eps)
Shirley Stoler
Mrs. Steve (13 eps)
Gregory Harrison
Conky 2000 (voice) / Knucklehead (voice) (13 eps)
Natasha Lyonne
Opal (13 eps)
Diane Yang
Cher (13 eps)
Alisan Porter
Li'l Punkin (2 eps)
Johann Carlo
Dixie (2 eps)
Shaun Weiss
Elvis (2 eps)
Roland Rodriquez
Tito (1 eps)
Leslie Jordan
Bubsy (1 eps)
Max Robert
Organist (1 eps)