Tara Strong
Twilight Sparkle (voice) (26 eps)
Andrea Libman
Pinkie Pie / Fluttershy (voice) (26 eps)
Ashleigh Ball
Rainbow Dash / Applejack (voice) (26 eps)
Tabitha St. Germain
Rarity / Princess Luna / Granny Smith / Mrs. Cake (voice) (26 eps)
Cathy Weseluck
Spike / Mayor Mare (voice) (26 eps)
Michelle Creber
Apple Bloom (voice) (26 eps)
Claire Margaret Corlett
Sweetie Belle (voice) (26 eps)
Madeleine Peters
Scootaloo (voice) (26 eps)
Nicole Oliver
Princess Celestia / Cheerilee (voice) (26 eps)
Britt McKillip
Princess Cadance (voice) (26 eps)
Peter New
Big McIntosh (voice) (26 eps)
Brenda Crichlow
Zecora (voice) (26 eps)
Chantal Strand
Diamond Tiara (voice) (26 eps)
John de Lancie
Discord (voice) (26 eps)
Shannon Chan-Kent
Silver Spoon (voice) / Pinkie Pie (singing) (26 eps)
Brian Drummond
Mr. Cake (voice) (26 eps)
Rebecca Shoichet
Twilight Sparkle (singing) (26 eps)
Kazumi Evans
Rarity (singing) (26 eps)
Andrew Francis
Shining Armor (voice) (26 eps)
Ellen Kennedy
Mane-iac (voice) (2 eps)
Michael Dobson
Bulk Biceps (voice) (2 eps)
Ingrid Nilson
Maud Pie (voice) (2 eps)
Mark Acheson
Lord Tirek (voice) (2 eps)
'Weird Al' Yankovic
Cheese Sandwich (voice) (1 eps)
Alvin Sanders
Flutterguy (voice) (1 eps)
Sam Vincent
Flim (voice) (1 eps)
Scott McNeil
Flam (voice) (1 eps)
Ian James Corlett
Silver Shill (voice) (1 eps)
Vincent Tong
Flash Sentry / Dignitary (voice) (1 eps)
Jayson Thiessen
Rare Find (voice) (1 eps)
Chiara Zanni
Daring Do / A.K. Yearling (voice) (1 eps)
Kelly Metzger
Spitfire / Blossomforth (voice) (1 eps)
Sylvain LeVasseur-Portelance
Stellar Eclipse (voice) (1 eps)
Saffron Henderson
Daring Do Collector / Pony 2 (voice) (1 eps)
James Higuchi
Comic Geek Pony / Pony 1 (voice) (1 eps)
Michael Daingerfield
Antique Chicken Stand Pony / Ancient Beast Dealer (voice) (1 eps)