Drake Bell
Peter Parker / Spider-Man (voice) (103 eps)
Misty Lee
Aunt May Parker (voice) (103 eps)
J.K. Simmons
J. Jonah Jameson (voice) (103 eps)
Ogie Banks
Luke Cage / Power Man (voice) (103 eps)
Chi McBride
Nick Fury (voice) (78 eps)
Tyler James Williams
Prowler (voice) (52 eps)
Clark Gregg
Phil Coulson (voice) (52 eps)
Caitlyn Taylor Love
Ava Ayala / White Tiger (voice) (52 eps)
Tom Kenny
Dr. Otto Octavius / Bentley Wittman (voice) (52 eps)
Dante Basco
Flash Thompson (voice) (52 eps)
Greg Cipes
Danny Rand / Iron Fist (voice) (52 eps)
Matt Lanter
Harry Osborn (voice) (52 eps)
Logan Miller
Sam Alexander / Nova (voice) (52 eps)
Mark Hamill
Arnim Zola (voice) (7 eps)
Ashley Eckstein
Dagger (voice) (6 eps)
Milo Ventimiglia
Spider-Man Noir (voice) (4 eps)
Imari Williams
Bone Spider/Goliath Spider (voice) (2 eps)
Ross Lynch
Jack Russell / Werewolf by Night (voice) (1 eps)
Cam Clarke
Captain Ultra/Piledriver (voice) (1 eps)
James Marsters
Korvac/Chitauri #3 (voice) (1 eps)
Billy West
Rocket Raccoon (voice) (1 eps)
Robert Clotworthy
Captain George Stacy (voice) (1 eps)
Max Mittelman
Alex O'Hirn / Rhino (voice) (1 eps)
Steve Blum
Wolverine (voice) (1 eps)
Peter Lurie
Sabretooth/Construction Worker/Announcer (voice) (1 eps)
Grant George
Scott Lang / Ant-Man (voice) (1 eps)
Debby Ryan
Jessie Prescott (voice) (1 eps)
Peyton List
Emma Ross (voice) (1 eps)
Cameron Boyce
Luke Ross (voice) (1 eps)
Karan Brar
Ravi Ross (voice) (1 eps)
Skai Jackson
Zuri Ross (voice) (1 eps)
Seth Green
Rick Jones / A-Bombardier (voice) (1 eps)
Dove Cameron
Gwen Stacy, Spider-Gwen (voice) (1 eps)
Christopher Daniel Barnes
Wolf Spider, Spyder-Knight (voice) (1 eps)
Cree Summer
Madame Web (voice) (1 eps)
Ben Diskin
Blood Spider (voice) (1 eps)