Robbie Daymond
Peter Parker / Spider-Man (voice) (6 eps)
Nadji Jeter
Miles Morales (voice) (6 eps)
Fred Tatasciore
Max Modell (voice) (6 eps)
Laura Bailey
Gwen Stacy (voice) (6 eps)
Melanie Minichino
Anya Corazon (voice) (6 eps)
Nancy Linari
Aunt May (voice) (6 eps)
Patton Oswalt
Uncle Ben (voice) (6 eps)
Scott Menville
Doc Ock / Otto Octavius (voice) (6 eps)
Jim Cummings
Hammerhead (voice) (6 eps)
Ben Diskin
Flash Thompson (6 eps)
Josh Keaton
Norman Osborn (voice) (6 eps)
John DiMaggio
Raymond Warren (voice) (6 eps)
Alastair Duncan
Adrian Toomes (voice) (6 eps)
Max Mittelman
Harry Osborn (voice) (6 eps)