Fred Tatasciore
Max Modell (voice) (62 eps)
Melanie Minichino
Anya Corazon (voice) (61 eps)
Ben Diskin
Flash Thompson (61 eps)
Nancy Linari
Aunt May (voice) (60 eps)
Max Mittelman
Harry Osborn (voice) (60 eps)
Laura Bailey
Gwen Stacy (voice) (60 eps)
Josh Keaton
Norman Osborn (voice) (60 eps)
Nadji Jeter
Miles Morales (voice) (59 eps)
Robbie Daymond
Peter Parker / Spider-Man (voice) (59 eps)
John DiMaggio
Raymond Warren (voice) (59 eps)
Scott Menville
Doc Ock / Otto Octavius (voice) (59 eps)
Alastair Duncan
Adrian Toomes (voice) (58 eps)
Patton Oswalt
Uncle Ben (voice) (57 eps)
Jim Cummings
Hammerhead (voice) (57 eps)
Jason Spisak
Alistair Smythe (voice) (6 eps)
Aaron Abrams
Tinkerer (voice) (4 eps)
Sofia Carson
Sand Girl (voice) (3 eps)
Yuri Lowenthal
Nocturnal (voice) (3 eps)
Bob Joles
J. Jonah Jameson (voice) (3 eps)
Zeno Robinson
Randy Robertson (voice) (2 eps)
Matthew Mercer
Aleksei Sytsevich/Comic Book Guy (voice) (2 eps)
Grey DeLisle
Black Cat/Nurse/Mom (voice) (2 eps)
Cameron Boyce
Herman Schultz / Shocker (voice) (1 eps)
Nathaniel J. Potvin
Prowler (voice) (1 eps)
Richard Doyle
Police Officer #3 (voice) (1 eps)
Daniel Hagen
Cop (voice) (1 eps)
Travis Willingham
Sandman / Flint Marko (voice) (1 eps)
Jeremy Shada
Ross Caliban (voice) (1 eps)
Sumalee Montano
Yuri Watanabe (voice) (1 eps)
Felicia Day
Mary Jane (voice) (1 eps)
Alex Désert
Swarm (voice) (1 eps)
Yvette Nicole Brown
Head Administrator (voice) (1 eps)