Arleen Sorkin
Harley Quinn / Dr. Harleen Quinzel (voice) (31 eps)
Diane Pershing
Poison Ivy / Dr. Pamela Isley (voice) (31 eps)
Tara Strong
Batgirl / Barbara Gordon (voice) (31 eps)
Adrienne Barbeau
Catwoman / Selina Kyle (voice) (31 eps)
Tom Kenny
Cops (voice) (10 eps)
Jennifer Hale
Dora Smithy / Caroline Greenway (voice) (9 eps)
Dee Bradley Baker
Jonesy / Muggers (voice) (5 eps)
Bob Hastings
Commissioner Jim Gordon (voice) (4 eps)
Paul Dini
Man (voice) (2 eps)
Stacie Randall
Zatanna Zatara (voice) (2 eps)