Yuhko Kaida
Tsukuyo (voice) (368 eps)
Tomokazu Sugita
Gintoki Sakata (voice) (367 eps)
Rie Kugimiya
Kagura (voice) (367 eps)
Daisuke Sakaguchi
Shinpachi Shimura (voice) (367 eps)
Akira Ishida
Kotarou Katsura (voice) (367 eps)
Fumihiko Tachiki
Taizou Hasegawa (voice) (367 eps)
Kazuya Nakai
Toushirou Hijikata (voice) (367 eps)
Kenichi Suzumura
Sougo Okita (voice) (367 eps)
Takehito Koyasu
Shinsuke Takasugi (voice) (367 eps)
Satoshi Hino
Kamui (voice) (367 eps)
Susumu Chiba
Kondou, Isao (voice) (367 eps)
Kujira
Otose (voice) (367 eps)
Mikako Takahashi
Sadaharu (voice) (367 eps)
Kazuhiko Inoue
Oboro (76 eps)
Shin-ichiro Miki
Sakamoto, Tatsuma (voice) (50 eps)
Hochu Otsuka
Abuto (voice) (49 eps)
Keikou Sakai
Agonoske, Azumi (voice) (49 eps)
Yuu Sugimoto
Catherine (voice) (49 eps)
Shinji Takamatsu
Elizabeth (voice) (49 eps)
Junichi Suwabe
Goemon (voice) (49 eps)
Kana Ueda
Hanako (voice) (49 eps)
Keiji Fujiwara
Hattori, Zenzou (voice) (49 eps)
Tessyo Genda
Hedoro (voice) (49 eps)
Takeshi Aono
Hiraga, Gengai (voice) (49 eps)
Show Hayami
Kankou (voice) (49 eps)
Takumi Yamazaki
Kawakami, Bansai (voice) (49 eps)
Sayaka Narita
Ketsuno, Christel (voice) (49 eps)
Norio Wakamoto
Matsudaira, Katakuriko (voice) (49 eps)
Hisao Egawa
Saigou, Tokumori (voice) (49 eps)
Akio Otsuka
Saizou (voice) (49 eps)
Satsuki Yukino
Shimura, Tae (voice) (49 eps)
Hideaki Sorachi
Sorachi, Hideaki (voice) (49 eps)
Fumiko Orikasa
Yagyuu, Kyuubei (voice) (49 eps)
Koichi Yamadera
Yoshida, Shouyou (voice) (49 eps)
Iku Minase
Ichiro (voice) (2 eps)
Akeno Watanabe
Mutsu (voice) (1 eps)
Yuuko Ueda
Grandma (voice) (1 eps)
Yuichi Karasuma
Slave Ship Leader (voice) (1 eps)
Rei Yamahata
Pirate A (voice) (1 eps)
Yoshihito Sasaki
Pirate B (voice) (1 eps)