Nick Kroll
Nick Birch / Maury the Hormone Monster / Coach Steve (voice) (71 eps)
John Mulaney
Andrew Glouberman (voice) (71 eps)
Jessi Klein
Jessi Glaser (voice) (71 eps)
Jason Mantzoukas
Jay Bilzerian (voice) (71 eps)
Maya Rudolph
Diane Birch / Constance the Hormone Monstress (voice) (71 eps)
Fred Armisen
Elliot Birch (voice) (71 eps)
Jordan Peele
Ghost of Duke Ellington (voice) (71 eps)
Andrew Rannells
Matthew MacDell (voice) (52 eps)
Jenny Slate
Missy Foreman-Greenwald (voice) (41 eps)
Ayo Edebiri
Missy Foreman-Greenwald (voice) (32 eps)
Joe Wengert
Caleb (voice) (23 eps)
Richard Kind
Marty Glouberman (voice) (23 eps)
Paula Pell
Barbara Glouberman (voice) (20 eps)
Neil Casey
Lars (voice) (19 eps)
Jak Knight
DeVon (voice) (19 eps)
June Diane Raphael
Devin (voice) (18 eps)
Jon Daly
Judd Birch (voice) (17 eps)
Kat Dennings
Leah Birch (voice) (15 eps)
Seth Morris
Greg Glaser (voice) (15 eps)
Gina Rodriguez
Gina Alvarez (voice) (14 eps)
Chelsea Peretti
Monica Foreman-Greenwald (voice) (12 eps)
Brandon Kyle Goodman
Walter the Lovebug (voice) (11 eps)
Heather Lawless
Jenna Bilzerian (voice) (11 eps)
Mark Rivers
(voice) (10 eps)
Mark Duplass
Val Bilzerian (voice) (10 eps)
Gil Ozeri
Brad (voice) (9 eps)
David Thewlis
Shame Wizard (voice) (8 eps)
Zachary Quinto
Aiden (voice) (8 eps)
Nathan Fillion
Nathan Fillion (voice) (7 eps)
Paul Scheer
Kurt Bilzerian (voice) (7 eps)
Emily Altman
Milk (voice) (6 eps)
Rob Huebel
Mr. Lizer (voice) (6 eps)
Ali Wong
Ali (voice) (6 eps)
Maria Bamford
Tito the Anxiety Mosquito (voice) (6 eps)
Jean Smart
Depression Kitty (voice) (6 eps)
John Gemberling
Tyler the Hormone Monster (voice) (5 eps)
Michaela Watkins
Cantor Dina Reznick (voice) (5 eps)
Fran Gillespie
(voice) (5 eps)
Jack McBrayer
Pubic Hair #1 (voice) (5 eps)
Kristen Bell
Pam the Pillow (voice) (4 eps)
Craig Robinson
Pubic Hair #2 (voice) (4 eps)
Ashley London
Kitty / DNA Expert / Pornscape Residents (voice) (4 eps)
Rosa Salazar
Ms. Benitez (voice) (4 eps)
Gary Cole
Matthew's Father (voice) (4 eps)
Thandiwe Newton
Mona (voice) (4 eps)
Ayden Mayeri
Gabrielle / Freshman (voice) (4 eps)
Natasha Lyonne
Suzette (voice) (3 eps)
Andy Daly
Ghost of Harry Houdini (voice) (3 eps)
Bobby Cannavale
Nick's Agent (voice) (3 eps)
John Oliver
Harry (voice) (3 eps)
Seth Rogen
Seth Goldberg (voice) (3 eps)
Julie White
Kimberly (voice) (3 eps)
Sterling K. Brown
Michael Angelo (voice) (3 eps)
Quinta Brunson
Quinta (voice) (3 eps)
Kristen Wiig
Jessi's Vagina (voice) (3 eps)
Stephanie Beatriz
Lulu (voice) (3 eps)
Megan Thee Stallion
Megan the(e?) Hormone Monstress (voice) (3 eps)
Zach Woods
Sock (voice) (2 eps)
Ileen Goldsmith
Jessi Glaser - singing double / Judd's Girlfriend (voice) (2 eps)
Brendan McCreary
Freddie Mercury (singing voice) (2 eps)
Carlos Alazraqui
Gustavo (voice) (2 eps)
Julie Klausner
Cherry Marashina (voice) (2 eps)
Chris Redd
(voice) (2 eps)
Josie Totah
Natalie (voice) (2 eps)
Lena Waithe
Lena (voice) (2 eps)
Joel Kim Booster
Charles Lu (voice) (2 eps)
Judd Hirsch
Lewis Glouberman (voice) (2 eps)
Chloe Fineman
Leah Birch (voice) (2 eps)
Patrick Page
Dread (voice) (2 eps)
Beck Bennett
Owen (voice) (2 eps)
J Valerione
B-High Qee-Choi Member (voice) (2 eps)
Jon Hamm
Scallops (voice) (1 eps)
Jon Gabrus
Movie Theater Customer (voice) (1 eps)
Anna Graves
Mrs. Albert (voice) (1 eps)
Nasim Pedrad
Fatima (voice) (1 eps)
Mae Whitman
Tallulah Levine (voice) (1 eps)
Chrissy Guerrero
Choir Girl (voice) (1 eps)
Philip Quinaz
Tanya Alvarez (voice) (1 eps)
Harvey Fierstein
Older Guy Town Resident (voice) (1 eps)
Jerry Minor
Man #3 / Singing Fish (voice) (1 eps)
Piotr Michael
Siamese Twin #2 (voice) (1 eps)
Marisa Lark Wallin
Reese's Wertherspoon / Woman (voice) (1 eps)
Crissy Guerrero
(voice) (1 eps)
Martin Short
Gordie (voice) (1 eps)
David Cross
Skip Glouberman (voice) (1 eps)
Carol Kane
Menopause Banshee / Old Woman (voice) (1 eps)
Tawny Newsome
(voice) (1 eps)
Retta
Duke's Mother (voice) (1 eps)
Wanda Sykes
Ghost of Harriet Tubman (voice) (1 eps)
Jaboukie Young-White
(voice) (1 eps)
Rob Riggle
Sgt. Adderall (voice) (1 eps)
Bobby Berk
Bobby Berk (voice) (1 eps)
Karamo Brown
Karamo Brown (voice) (1 eps)
Tan France
Tan France (voice) (1 eps)
Antoni Porowski
Antoni Porowski (voice) (1 eps)
Jonathan van Ness
Jonathan Van Ness (voice) (1 eps)
Anna Konkle
Izzy (voice) (1 eps)
Maya Erskine
Misha (voice) (1 eps)
Alia Shawkat
Roland (voice) (1 eps)
Paul Giamatti
Andrew’s Shit (voice) (1 eps)
Adam Scott
Mr. Keating (voice) (1 eps)
Hugh Jackman
himself (voice) (1 eps)
Kumail Nanjiani
himself (voice) (1 eps)
Keke Palmer
Rochelle the Hate Worm (voice) (1 eps)
Jemaine Clement
Simon Sex (voice) (1 eps)
Kristen Schaal
Bernie Sanders (voice) (1 eps)
Aidy Bryant
Emmy (voice) (1 eps)
Somali Rose
Missy’s Overalls (1 eps)
Billy Porter
Ocean (voice) (1 eps)
Rosie Perez
Petra (voice) (1 eps)
Lupita Nyong'o
Asha (voice) (1 eps)
Miranda Tapsell
Kara (voice) (1 eps)
Lin-Manuel Miranda
Puerto Rican Pubic Hair (voice) (1 eps)
Randall Park
Pete Doheny (voice) (1 eps)
Maitreyi Ramakrishnan
Marissa (voice) (1 eps)
Ava Lalezarzadeh
Nazanin (voice) (1 eps)
Don Cheadle
Don Cheadle (voice) (1 eps)
Padma Lakshmi
Priya (voice) (1 eps)
Zach Galifianakis
Gratitoad (voice) (1 eps)