Kate Beckinsale
Selene
Stephen Rea
Dr. Jacob Lane
Michael Ealy
Detective Sebastian
Theo James
David
India Eisley
Eve
Charles Dance
Thomas
Sandrine Holt
Lida
Sanny van Heteren
British newscaster
Jacob Blair
Kolb (Officer)
Robert Lawrenson
Waterfront Cop
Kurt Max Runte
Leader of the Troop
Marvin Duerkholz
Lestroy
Ron Wear
Jack Fletcher
Richard Cetrone
Lycan 1
Dan Payne
Lycan 2
Mark Gibbon
Announcing Guard
Benita Ha
Surgical Nurse
Tyler McClendon
Scientist
Panou
Old City Cop #1
Ian Rozylo
Old City Cop #2
Kristen Holden-Ried
Quint Lane
John Innes
Medical Supervisor
Lee Majdoub
Desk Guard
Catlin Adams
Olivia
Adam Greydon Reid
Med Tech
Christian Tessier
Security Guard #1
Wes Bentley
Antigen Scientist (uncredited)
Scott Speedman
Michael (archive footage) (uncredited)
Joe Censoplano
Cadillac Driver (uncredited)
Preston Cook
Antigen Surgeon (uncredited)