Fay Compton
Mary Stuart
Gerald Ames
Bothwell
Ivan Samson
Lord Dudley
John Stuart
George Douglas
Ellen Compton
Queen Elizabeth
Lionel d'Aragon
Moray
Harvey Braban
Ruthven
Irene Rooke
Catherine de Medici
Donald Macardle
Francis II
René Maupré
Rizzio
Ernest A. Douglas
Cardinal
Sydney Seaward
Lord Douglas
Edward Sorley
John Knox
Betty Faire
Mary Beaton
Dorothy Fane
Mary Beaton
Nancy Kenyon
Mary Fleming
Julie Hartley-Milburn
Mary Seaton
Basil Rathbone
Undetermined Subordinate Role