Richard Greene
Sir Henry Baskerville
Basil Rathbone
Sherlock Holmes
Wendy Barrie
Beryl Stapleton
Nigel Bruce
Doctor John H. Watson
Lionel Atwill
Dr. James Mortimer
John Carradine
Barryman
Barlowe Borland
Frankland
Beryl Mercer
Mrs. Jennifer Mortimer
Morton Lowry
John 'Jack' Stapleton
Ralph Forbes
Sir Hugo Baskerville
E. E. Clive
London Cabbie John Clayton
Eily Malyon
Mrs. Barryman
Lionel Pape
The Coroner
Nigel De Brulier
The Notting Hill Murderer
Mary Gordon
Mrs. Hudson
Ian Maclaren
Sir Charles Baskerville
Ruth Terry
Betsy Ann
Mary Young
Betsy Ann's Mother (uncredited)
Kenneth Hunter
Ship's Officer (uncredited)
Vesey O'Davoren
Ship's Steward (uncredited)
John Graham Spacey
Ship's Porter (uncredited)
David Thursby
Open Carriage Driver (uncredited)
Peter Willes
Roderick
Leonard Carey
Hugo's Servant (uncredited)
Rita Page
Chambermaid (uncredited)
Jack Egger
(uncredited)
Frank McLure
Ship Passenger (uncredited)
Brandon Beach
Ship Passenger (uncredited)
Blitzen
The Hound (as Chief)
James Carlisle
Ship Passenger (uncredited)
Herschel Graham
Ship Passenger (uncredited)