Barrie Ingham
Basil of Baker Street / Bartholomew (voice)
Val Bettin
Dr. David Q. Dawson (voice)
Vincent Price
Professor Ratigan (voice)
Susanne Pollatschek
Olivia Flaversham (voice)
Candy Candido
Fidget (voice)
Diana Chesney
Mrs. Judson (voice)
Eve Brenner
The Mouse Queen (voice)
Alan Young
Hiram Flaversham (voice)
Basil Rathbone
Sherlock Holmes (voice)
Ellen Fitzhugh
Bar Maid (voice)
Walker Edmiston
Citizen (voice)
Wayne Allwine
Thug Guard (voice)
Tony Anselmo
Thug Guard (voice)
Frank Welker
Toby the Dog / Felicia the Cat (voice) (uncredited)
Laurie Main
Dr. Watson (archive sound)
Shani Wallis
Lady Mouse (voice)
Melissa Manchester
Miss Kitty Mouse (voice)
Charles Fleischer
Additional Voices (voice) (uncredited)