Cedric Hardwicke
Dr. Ludwig Frankenstein / Dr. Henry Frankenstein (ghostly image)
Ralph Bellamy
Erik Ernst
Lionel Atwill
Dr. Bohmer
Bela Lugosi
Ygor
Evelyn Ankers
Elsa Frankenstein
Lon Chaney Jr.
The Frankenstein Monster
Janet Ann Gallow
Cloestine Hussman
Barton Yarborough
Dr. Kettering
Doris Lloyd
Martha the Housekeeper
Leyland Hodgson
Chief Constable
Olaf Hytten
Adolph Hussman
Holmes Herbert
Magistrate
Richard Alexander
Villager at Council Meeting (uncredited)
Lionel Belmore
Councilman (uncredited)
Chet Brandenburg
Villager (uncredited)
Colin Clive
Dr. Henry Frankenstein (flashback) (uncredited)
Harry Cording
Mob Leader (uncredited)
George Eldredge
Constable (uncredited)
Dwight Frye
Villager at Meeting / Grave Robber (flashback) (uncredited)
Lawrence Grant
Mayor (uncredited)
Otto Hoffman
Villager (uncredited)
Brandon Hurst
Hans, Villager at Council Meeting (uncredited)
Michael Mark
Councilman (uncredited)
Jimmy Phillips
Boy Playing Indian (uncredited)
William Smith
Boy (uncredited)
Ernie Stanton
Constable (uncredited)
Julius Tannen
Sektal (uncredited)
Harry Tenbrook
Villager at Hearing (uncredited)
Glen Walters
Mother of Hungry Children (uncredited)
Janet Warren
Goose-herd (uncredited)
Teddy Infuhr
Boy (uncredited)