Boris Karloff
The Monster
Ernest Thesiger
Doctor Pretorius
Colin Clive
Henry Frankenstein
O. P. Heggie
Hermit
Una O'Connor
Minnie
Valerie Hobson
Elizabeth
Dwight Frye
Karl
Elsa Lanchester
Mary Wollstonecraft Shelley / The Monster's Bride
Ted Billings
Ludwig
E. E. Clive
Burgomaster
Gavin Gordon
Lord Byron
Douglas Walton
Percy Bysshe Shelley
Reginald Barlow
Hans
Lucien Prival
Butler
Mary Gordon
Hans' Wife
Anne Darling
Shepherdess
Billy Barty
Baby (uncredited)
Robert Adair
Hunter in Woods (uncredited)
Norman Ainsley
Archbishop (uncredited)
Frank Benson
Villager (uncredited)
Maurice Black
Gypsy (uncredited)
Walter Brennan
Peasant (uncredited)
Mae Bruce
Villager (uncredited)
A.S. Byron
King Homunculus (uncredited)
John Carradine
Lost Hunter at Hermit's Cottage (uncredited)
D'Arcy Corrigan
Procession Leader (uncredited)
Grace Cunard
Villager (uncredited)
J. Gunnis Davis
Uncle Glutz (uncredited)
Marie DeForrest
Ballerina (uncredited)
Elspeth Dudgeon
Gypsy's Mother (uncredited)
Helen Jerome Eddy
Gypsy's Wife (uncredited)
Neil Fitzgerald
Rudy (uncredited)
Brenda Fowler
Mother (uncredited)
John George
Villager (uncredited)
Helen Gibson
Villager (uncredited)
Marilyn Harris
Girl (uncredited)
Carmencita Johnson
Friend of Murdered Girl (uncredited)
Rollo Lloyd
Neighbor (uncredited)
Murdock MacQuarrie
Sympathetic Villager (uncredited)
Josephine McKim
Little Mermaid (uncredited)
Torben Meyer
Victim in flashback (uncredited)
Edwin Mordant
Coroner (uncredited)
Charles Murphy
Guard (uncredited)
Joseph North
Servant (uncredited)
Helen Parrish
Communion Girl (uncredited)
Edward Peil Sr.
Villager (uncredited)
Tempe Pigott
Auntie Glutz (uncredited)
Sarah Schwartz
Marta (uncredited)
Peter Shaw
Devil (uncredited)
Mary Stewart
Neighbor (uncredited)
Frank Terry
Lost Hunter at Hermit's Cottage (uncredited)
Anders Van Haden
Villager (uncredited)
Dorothy Vernon
Maid (uncredited)
Lucio Villiegas
Priest (uncredited)
Joan Woodbury
Queen Homunculus (uncredited)