Keanu Reeves
Thomas "Neo" Anderson (voice)
Carrie-Anne Moss
Trinity (voice)
Clayton Watson
The Kid (voice)
Pamela Adlon
Jue ("Final Flight of the Osiris") / Manabu ("Beyond") (voice)
Melinda Clarke
Alexa ("Matriculated") (voice)
John DiMaggio
Crew Man ("Final Flight of the Osiris") / Kaiser ("Program") (voice)
Kevin Michael Richardson
Thadeus ("Final Flight of Osiris") / Cop ("Kid's Story") / Agent #2 ("World Record")
Tom Kenny
Operator ("Final Flight of Osiris")
Rick Gomez
Pilot ("Final Flight of Osiris")
Tara Strong
Crew Woman ("Final Flight of Osiris") / Nurse ("World Record") / Misha ("Beyond")
Bette Ford
Old Woman ("Final Flight of Osiris")
Julia Fletcher
The Instructor ("The Second Renaissance Part I & II") / Narrator ("World Record")
Dane A. Davis
01 Versatran Spokesman ("The Second Renaissance Part I & II")
Debi Derryberry
Kid ("The Second Renaissance Part I & II")
Jill Talley
Mother ("The Second Renaissance Part I & II")
Dwight Schultz
Additional Voices ("The Second Renaissance Part I & II") / Monaka ("Matriculated")
James Arnold Taylor
Additional Voices ("The Second Renaissance Part I & II") / Ash ("A Detective Story") / Raul ("Matriculated")
John DeMita
Teacher ("Kid's Story")
Hedy Burress
Cis ("Program") / Yoko ("Beyond")
Phil LaMarr
Duo ("Program")
Victor Williams
Dan ("World Record")
John Wesley
Dan's Dad ("World Record")
Alex Fernandez
Tom ("World Record")
Allison Smith
Reporter ("World Record")
Matt McKenzie
Agent #1 ("World Record") / Agent ("Beyond") / Agent ("A Detective Story")
Tress MacNeille
Housewife ("Beyond") / Kenny ("Beyond")
Kath Soucie
Pudgy ("Beyond") / Masa ("Beyond") / Sara ("Beyond")
Terrence 'T.C.' Carson
Clarence ("A Detective Story")
Rodney Saulsberry
Chyron ("Matriculated")
Olivia d'Abo
Rox ("Matriculated")
Jack Fletcher
Sandro ("Matriculated")
Akio Otsuka
Thadeus (voice)