Basil Rathbone
Sherlock Holmes
Nigel Bruce
Dr. John H. Watson
Alan Mowbray
Major Duncan-Bleek
Dennis Hoey
Inspector Lestrade
Renee Godfrey
Vivian Vedder
Frederick Worlock
Prof. William Kilbane
Mary Forbes
Lady Margaret Carstairs
Skelton Knaggs
Sands - Moran's Accomplice
Billy Bevan
Conductor Taking Tickets
Geoffrey Steele
Roland Carstairs
Jane Murdoch
Mrs. Shallcross (uncredited)
Gerald Hamer
Alf Shallcross (uncredited)
Leyland Hodgson
Senior Conductor (uncredited)
Harry Cording
Mock the Undertaker (uncredited)
Charles Knight
Baggage Car Guard (uncredited)
Boyd Davis
"Inspector MacDonald" (uncredited)
Bobby Wissler
Mock Jr. (uncredited)
Gilbert Allen
Dining Car Steward (uncredited)
C. Aubrey Smith
Elderly gentleman on train station (uncredited)