Milla Jovovich
Alice
Oded Fehr
Carlos Olivera
Ali Larter
Claire Redfield
Iain Glen
Dr. Isaacs
Ashanti
Betty
Christopher Egan
Mikey
Spencer Locke
K-Mart
Matthew Marsden
Slater
Linden Ashby
Chaser
Jason O'Mara
Albert Wesker
Mike Epps
L.J.
Joe Hursley
Otto
James Tumminia
Lab Tech
Madeline Carroll
White Queen
Carrie Eklund
Camp Survivor (uncredited)
Brian Steele
Rancid / Tyrant
John Eric Bentley
Umbrella Tech
Kirk B.R. Woller
Scientist
Rick Cramer
Ice Hockey / Corridor Guard
Peter O'Meara
British Envoy
William Abadie
French Envoy
Ramón Franco
Runty
Shane Woodson
Piggy
Geoff Meed
Pock Mark
Rusty Joiner
Eddie
Connor McCoy
Small Boy
Carolina Jiménez
Mirror Woman
Gary A. Hecker
Tyrant Vocal (voice)
Martin Crewes
Chad Kaplan (uncredited)
Richard Epcar
Additional Voices (voice) (uncredited)
Stefen Hayes
Yuri Loginova (uncredited)
Gary Hudson
Umbrella Captain (uncredited)
Ryan McCluskey
Mr. Grey (uncredited)
Carsten Norgaard
Zombie Steve (uncredited)
César Ámigo Aguilar
Foreign Envoy (uncredited)