Tara Strong
Sci-Twi / Midnight Sparkle / Twilight Sparkle (voice)
Rebecca Shoichet
Sunset Shimmer (voice) / Sci-Twi (singing voice)
Ashleigh Ball
Applejack / Rainbow Dash (voice)
Andrea Libman
Pinkie Pie / Fluttershy / Bon Bon (voice)
Tabitha St. Germain
Rarity / Vice Principal Luna (voice)
Cathy Weseluck
Spike (voice)
Iris Quinn
Principal Abacus Cinch (voice)
Nicole Oliver
Principal Celestia (voice)
Britt McKillip
Dean Cadance (voice)
Vincent Tong
Flash Sentry / Bus Driver (voice)
Andrew Francis
Shining Armor (voice)
Sienna Bohn
Sugarcoat (voice)
Sharon Alexander
Sour Sweet (voice)
Kelly Sheridan
Indigo Zap (voice)
Britt Irvin
Sunny Flare (voice)
Shannon Chan-Kent
Lemon Zest (voice) / Pinkie Pie (singing voice)
Kazumi Evans
Rarity (singing voice)
Lili Beaudoin
Tennis Match (voice)
Diana Kaarina
Mystery Mint (voice)
Ingrid Nilson
Sweet Leaves (voice)
Kaylee Johnston
Background singer (voice uncredited)