Amy Nicholson
Host (Chapter 1: 'Wind') (voice)
Rodney Ascher
Host (Chapter 2: 'Membranes') (voice)
John Waters
Host (Chapter 3: 'Kindred') (voice)
Karyn Kusama
Host (Chapter 4: 'Multitudes') (voice)
Justin Benson
Host (Chapter 5: 'Judy') (voice)
Aaron Moorhead
Host (Chapter 5: 'Judy') (voice)
David Lowery
Host (Chapter 6: 'Dig') (voice)
David Lynch
Self (archive footage)
Jason Stoval
Lounge Wizard (as Sid Pink)
Judy Garland
Self (archive footage)
Jay Leno
Self (archive footage)
Jack Paar
Self (archive footage)