Tyrone Power
Jesse Woodson James
Henry Fonda
Frank James
Nancy Kelly
Zerelda "Zee" Cobb
Randolph Scott
Marshall Will Wright
Henry Hull
Major Rufus Cobb
Slim Summerville
Jailer
J. Edward Bromberg
George Runyan
Brian Donlevy
Barshee
John Carradine
Bob Ford
Donald Meek
McCoy
Johnny Russell
Jesse James Jr.
Jane Darwell
Mrs. Samuels - Jesse's mother
Charles Tannen
Charles Ford
Claire Du Brey
Mrs. Bob Ford
Willard Robertson
Clarke
Harold Goodwin
Bill
Ernest Whitman
Pinkie
Eddy Waller
Deputy
Paul E. Burns
Hank
Spencer Charters
Minister
Arthur Aylesworth
Tom Colson
Charles Middleton
Doctor
Charles Halton
Heywood
George Chandler
Roy
Harry Tyler
Farmer
Virginia Brissac
Boy's Mother
Edward LeSaint
Judge Rankin
John Elliott
Judge Mathews
Erville Alderson
Old Marshall
George P. Breakston
Farmer Boy
Lon Chaney Jr.
One Of James Gang
Carol Adams
Minor Role (uncredited)
Donald Douglas
Infantry Captain (uncredited)
James Flavin
Cavalry Captain (uncredited)
Sam Garrett
Rider / Roper (uncredited)
Wylie Grant
Barshee's Henchman
Harry Holman
Engineer (uncredited)
Kenner G. Kemp
Union Soldier (uncredited)
Leonard Kibrick
Boy (uncredited)
Sidney Kibrick
Boy (uncredited)
Ethan Laidlaw
Barshee's Henchman (uncredited)
Tom London
Soldier (uncredited)
George O'Hara
Teller (uncredited)
Paul Sutton
Lynch - Barshee's Henchman (uncredited)