Troy Baker
Bruce Wayne / Batman / The Joker (voice)
Eric Bauza
Leonardo (voice)
Darren Criss
Raphael (voice)
Kyle Mooney
Michelangelo (voice)
Baron Vaughn
Donatello (voice)
Rachel Bloom
Barbara Gordon / Batgirl (voice)
Ben Giroux
Damian Wayne / Robin (voice)
Brian George
Alfred Pennyworth (voice)
Jim Meskimen
Comissioner Gordon / Jonathan Crane / Scarecrow (voice)
Tom Kenny
Oswald Chesterfield Cobblepot / The Penguin (voice)
John DiMaggio
Victor Fries / Mr. Freeze (voice)
Tara Strong
Harleen F. Quinzel / Harley Quinn / Pamela Isley / Poison Ivy (voice)
Carlos Alazraqui
Bane (voice)
Cas Anvar
Ra's al Ghul (voice)
Keith Ferguson
Harvey Dent / Two-Face / Baxter Stockman (voice)
Andrew Kishino
Shredder (voice)